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Blood oxygen transport and tissue oxygenation were studied in 28 calves from the Belgian White and Blue breed (20 healthy and 8 hypoxaemic ones). Hypoxaemic calves were selected in response to their excessive respiratory frequency and to their low partial oxygen strain (PaO2) in the arterial blood. Venous and arterial blood samples have been collected, and 2,3-diphosphoglycerate, adenosine triphosphate, chloride, inorganic phosphate and hemoglobin concentrations, and pH, PCO, and PO2 were decided. An oxygen equilibrium curve (OEC) was measured in customary situations, for every animal. The arterial and venous OEC have been calculated, taking physique temperature, pH and PCO2 values in arterial and venous blood into account. The oxygen change fraction (OEF%), corresponding to the diploma of blood desaturation between the arterial and the venous compartments, and the quantity of oxygen launched at the tissue level by 100 mL of blood (OEF Vol%) had been calculated from the arterial and venous OEC mixed with the PO2 and hemoglobin concentration. In hypoxaemic calves investigated in this examine, the hemoglobin oxygen affinity, measured below commonplace circumstances, was not modified. Quite the opposite, in vivo acidosis and hypercapnia induced a lower in the hemoglobin oxygen affinity in arterial blood, which mixed to the decrease in PaO2 led to a decreased hemoglobin saturation diploma in the arterial compartment. However, this didn't impair the oxygen trade fraction (OEF%), since the hemoglobin saturation degree in venous blood was additionally diminished.
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